High capacity of purified mesenchymal stem cells for cartilage regeneration

• Grace Suto Eriko

  Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Mabuchi Yo

  Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Suzuki Nobuharu

  Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Koyanagi Asuka

  Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Kawabata Yoshiko

  Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Ogata Yusuke

  Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Ozeki Nobutake

  Department of Joint Surgery and Sports Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Nakagawa Yusuke

  Department of Joint Surgery and Sports Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Muneta Takeshi

  Department of Joint Surgery and Sports Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Sekiya Ichiro

  Department of Applied Regenerative Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

• Akazawa Chihiro

  Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan

抄録

Mesenchymal stem cells (MSCs) are a heterogeneous population of cells that proliferate and differentiate into bone, cartilage, and fat in vitro. Because of this multi-potency, the therapeutic applications of MSCs are under intensive exploration. The most common and redundant method for MSC cultivation requires prolonged culture on plastic dishes. The current study compared the differentiation/proliferative potency of purified mouse MSCs (CD45^-/ TER119^-/PDGFRα⁺/ Sca-1⁺ cells, or PαS cells) with whole bone marrow (WBM)-derived, plastic-adherent MSCs. After three passages, the surface expression levels of CD45, TER119, PDGFRα, and Sca-1 were evaluated in WBM and PαS cells. While PαS cells maintained high expression levels of both PDGFRα and Sca-1, WBM cells exhibited less expressed levels of these stem cell makers. Additionally, WBM cell cultures were frequently contaminated by CD45⁺ hematopoietic cells. Both cell migration and proliferation were significantly higher in PαS vs. WBM cells, indicating the enhanced differentiation potential of PαS cells for the mesenchymal lineage, and suggesting that WBM cell heterogeneity may regulate and limit the stemness of their MSC progeny. Consistent with this hypothesis, PαS cells transplanted locally at sites of cartilage defects displayed higher cartilage regeneration capacity than WBM cells in a rat osteochondral defect model. This is the first report to demonstrate its improved contribution to cartilage repair in vivo. Thus, the protocol employed for MSC isolation is crucial for the effective translation of MSC multi-potency into clinical therapeutics.

収録刊行物

• Inflammation and Regeneration

  Inflammation and Regeneration 35 (2), 078-085, 2015

  一般社団法人 日本炎症・再生医学会

キーワード

• bone marrow
• cartilage
• mesenchymal stem cell
• regeneration
• stem cell therapy

詳細情報

• CRID: 1390001205258191872
• NII論文ID: 130005063945
• DOI: 10.2492/inflammregen.35.078
• ISSN: 18808190
• ISSN: 18809693
• Web Site: https://search.jamas.or.jp/link/ui/2016090840
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可