Functional genetic analysis of <I>Arabidopsis thaliana SYNC1</I> in <I>Lotus corniculatus</I> super-growing roots using the FOX gene-hunting system

• Yano Tsubasa

  Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki These authors contributed equally to this work

• Tanaka Hidenori

  Faculty of Agriculture, University of Miyazaki These authors contributed equally to this work

• Kurino Taiki

  Graduate School of Agriculture, University of Miyazaki

• Yamamoto Akihiro

  Faculty of Agriculture, University of Miyazaki

• Kunitake Hisato

  Faculty of Agriculture, University of Miyazaki

• Saeki Yuichi

  Faculty of Agriculture, University of Miyazaki

• Akashi Ryo

  Faculty of Agriculture, University of Miyazaki

Abstract

To analyze the function of SYNC1, an Arabidopsis asparaginyl-tRNA synthetase gene, the FOX-hunting system using super-growing roots (SR) from the legume species Lotus corniculatus was employed. One transformed line, FSL#121, was compared to parental SR and to an SR line harboring the empty vector (Control), all of which were grown in vivo using vermiculite pots. The level of several free amino acids was higher in FSL#121 than SR. Concomitantly, FSL#121 had a distinct phenotype of greater shoot length, stem diameter and shoot fresh weight compared with SR. Also, the root length, root diameter and fresh root weight were greater in FSL#121 than SR. Furthermore, the greater number of nodules in FSL#121 increased the nitrogen fixation activity per whole plant. Therefore, SYNC1 overexpression caused distinct changes in plant growth, increased the number of root nodules, and may be involved in increasing the amount of free amino acids, especially asparagine.

Journal

• Plant Root

  Plant Root 9 (0), 6-14, 2014

  Japanese Society for Root Research

Keywords

• <I>Arabidopsis thaliana</I>
• FOX-SR line
• <I>Lotus corniculatus</I>
• <I>SYNC1</I>

Details

• CRID: 1390282680251641856
• NII Article ID: 130005064004
• DOI: 10.3117/plantroot.9.6
• ISSN: 18816754
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed