Polymorphisms of canine BRCA2 BRC repeats affecting interaction with RAD51
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- OCHIAI Kazuhiko
- Department of Veterinary Nursing and Technology, School of Veterinary Science, Nippon Veterinary and Life Science University
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- ISHIGURO-OONUMA Toshina
- Department of Biological Resources, Integrated Center for Science, Ehime University
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- YOSHIKAWA Yasunaga
- Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University
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- UDAGAWA Chihiro
- Department of Veterinary Nursing and Technology, School of Veterinary Science, Nippon Veterinary and Life Science University
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- KATO Yuiko
- Department of Veterinary Nursing and Technology, School of Veterinary Science, Nippon Veterinary and Life Science University
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- WATANABE Masami
- Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- BONKOBARA Makoto
- Department of Veterinary Clinical Pathology, School of Veterinary Science, Nippon Veterinary and Life Science University
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- MORIMATSU Masami
- Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
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- OMI Toshinori
- Department of Veterinary Nursing and Technology, School of Veterinary Science, Nippon Veterinary and Life Science University
書誌事項
- タイトル別名
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- <b>Polymorphisms of canine BRCA2 BRC repeats affecting interaction with </b><b>RAD51 </b>
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抄録
Mutations in the breast cancer susceptibility gene BRCA2 leading to the failure of interactions with the recombinase RAD51 are associated with an increased risk of cancer in humans. This interaction depends on the eight BRC repeat (BRC1–8) sequences in BRCA2. We previously reported that canine BRC3 has two polymorphisms (T1425P and K1435R) influencing the interaction with RAD51, and 1435R was identified in mammary tumor dog samples. In this study, we investigated the sequence variations of BRC3 and 4 in 236 dogs of five breeds. Allele frequencies of 1425P and 1435R were 0.063 and 0.314, respectively, and there was no other polymorphism in the sequenced region. A mammalian two-hybrid assay using BRC3–4 sequences demonstrated that 1425P allele reduced the binding strength with RAD51 but 1435R had no effect. These results may provide an insight into the functions of not only individual but also multiple BRC repeats of BRCA2 in dogs.
収録刊行物
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- Biomedical Research
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Biomedical Research 36 (2), 155-158, 2015
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