書誌事項
- タイトル別名
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- A Thin Polymer Membrane, the NanoSuit<Sup>®</Sup>, for the Observation of Living and Wet Organisms in High-Vacuum Scanning Electron Microscope
- ナノスーツホウ ニ ヨル イキタ ママ ノ セイブツ ノ SEM ナイ セイタイ カンサツ
- A Thin Polymer Membrane, the NanoSuit<Sup>®</Sup>, for the Observation of Living and Wet Organisms in High-Vacuum Scanning Electron Microscope
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抄録
Scanning electron microscopy (SEM) has become an essential tool for observing the biological materials, however, various complex procedures preclude observation of living organisms to date. Here we present a new method to observe living organisms by a SEM. We observed the active movements of animals in vacuo (10−5—10−7 Pa) by coating them with a thin polymer membrane, NanoSuit®, made by polyoxyethylene (20) sorbitan monolaurate and found that the surface fine structure of the living organism is very different from that of traditionally fixed samples. After observation of the larvae of mosquitos, despite the high vacuum, we could rear them in normal culture conditions. We also found that the NanoSuit® and self-standing biocompatible films were able to produce by plasma polymerization from various monomer solutions. Our method will be useful for numerous applications, particularly in the electron microscopic observation of life sciences.
収録刊行物
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- 表面科学
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表面科学 36 (4), 201-206, 2015
公益社団法人 日本表面科学会
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詳細情報 詳細情報について
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- CRID
- 1390282681436185472
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- NII論文ID
- 130005065849
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- NII書誌ID
- AN00334149
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- ISSN
- 18814743
- 03885321
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- NDL書誌ID
- 026342797
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可