Characterization of <i>Bombyx mori</i> Silk Fiber Incorporating an Unnatural Amino Acid (4-Chlorophenylalanine)

• Teramoto Hidetoshi

  独立行政法人農業生物資源研究所

• Nakajima Ken-ichi

  独立行政法人農業生物資源研究所

• Kojima Katsura

  独立行政法人農業生物資源研究所

Bibliographic Information

Other Title

• 非天然型アミノ酸（4-クロロフェニルアラニン）を導入した家蚕シルクの物性解析
• ヒテンネンガタ アミノサン(4-クロロフェニルアラニン)オ ドウニュウ シタ カサン シルク ノ ブッセイ カイセキ

Abstract

An unnatural amino acid, 4-chlorophenylalanine (ClPhe), was incorporated into silk fibroin of Bombyx mori silk fiber in place of a portion of the phenylalanine (Phe) residues using transgenic silkworms that can incorporate unnatural amino acids (4-substituted analogues of Phe) into protein biosynthesis in the posterior silk glands. Raw silk fiber containing ClPhe in silk fibroin was reeled from cocoons, and its characteristics, such as its tensile properties, were investigated. The thermal properties of the degummed fiber were also characterized. The results indicated that the partial replacement of Phe to ClPhe in silk fibroin did not affect the basic properties of Bombyx mori silk fiber.

Journal

• The Journal of Silk Science and Technology of Japan

  The Journal of Silk Science and Technology of Japan 23 (0), 27-35, 2015

  The Japanese Society of Silk Science and Technology

Keywords

• <i>Bombyx mori</i> silk fiber
• unnatural amino acid
• transgenic silkworm
• phenylalanine
• characterization
• tensile property

Details

• CRID: 1390001205273422720
• NII Article ID: 130005066407
• NII Book ID: AA11828182
• DOI: 10.11417/silk.23.27
• ISSN: 18811698
• ISSN: 18808204
• NDL BIB ID: 026309232
• Web Site: http://id.ndl.go.jp/bib/026309232
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026309232
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
  • Crossref
• Abstract License Flag: Disallowed