<I>In vivo</I> live-cell imaging in plant tissues by two-photon excitation microscopy

  • Mizuta Yoko
    Graduate School of Science, Nagoya University JST, ERATO, Higashiyama Live-Holonics Project, Nagoya University
  • Kurihara Daisuke
    Graduate School of Science, Nagoya University JST, ERATO, Higashiyama Live-Holonics Project, Nagoya University
  • Higashiyama Tetsuya
    Graduate School of Science, Nagoya University JST, ERATO, Higashiyama Live-Holonics Project, Nagoya University Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University

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Other Title
  • 2光子顕微鏡による植物深部の<I>in vivo</I>イメージング
  • 2光子顕微鏡による植物深部のin vivoイメージング
  • 2 ミツコ ケンビキョウ ニ ヨル ショクブツ シンブ ノ in vivo イメージング

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Abstract

In vivo live-cell imaging is an essential for understanding biological phenomena. In biological science, recent advances in imaging techniques using various fluorescence probes revealed dynamic cellular/extracellular events as they occur in real time at subcellular level. Two-photon excitation microscopy provides non-invasive tool for deep imaging in living organisms. We have been trying to deep imaging in plant tissues, using two-photon excitation microscopy to study the biological phenomena in vivo. In this review article, we summarize the characters of two-photon microscopy and advantages for deep imaging of plant tissues in vivo.

Journal

  • PLANT MORPHOLOGY

    PLANT MORPHOLOGY 26 (1), 25-30, 2014

    The Japanese Society of Plant Morphology

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