Clostridium perfringens TpeL Induces Formation of Stress Fibers via Activation of RhoA-ROCK Signaling Pathway

• Nagahama Masahiro

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Ohkubo Akiko

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Kinouchi Yoshihito

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Kobayashi Keiko

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Miyamoto Kazuaki

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Takehara Masaya

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Sakurai Jun

  Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

書誌事項

タイトル別名

• <i>Clostridium perfringens</i> TpeL Induces Formation of Stress Fibers <i>via</i> Activation of RhoA-ROCK Signaling Pathway

抄録

Clostridium perfringens TpeL belongs to a family of large clostridial glucosylating cytotoxins. TpeL modifies Rac1 and Ras subfamily proteins. Herein we report TpeL-induced formation of stress fibers via RhoA-Rho kinase (ROCK) signaling. A recombinant protein (TpeL1–525) derived from the TpeL N-terminal catalytic domain in the presence of streptolysin O (SLO) induced the formation of actin stress fibers in Madin–Darby canine kidney (MDCK) cells in a dose-dependent manner. The RhoA/ROCK pathway is known to control the formation of stress fibers. We examined the role of the RhoA/ROCK pathway in TpeL-induced formation of stress fibers. TpeL1–525-induced formation of stress fibers was inhibited by the ROCK inhibitor, Y27632 and Rho protein inhibitor, C3 transferase. TpeL1–525 activated RhoA and ROCK in a dose-dependent manner. C3 transferase blocked TpeL1–525-induced activation of RhoA and ROCK whereas Y27632 inhibited TpeL-induced activation of ROCK. These results demonstrate for the first time that TpeL induces the formation of stress fibers by activating the RhoA/ROCK signaling pathway.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 38 (5), 732-739, 2015

  公益社団法人 日本薬学会

キーワード

• <i>Clostridium perfringens</i> TpeL
• stress fiber
• RhoA
• Rho A-Rho kinase (ROCK)

詳細情報

• CRID: 1390282679609577984
• NII論文ID: 130005068100
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b14-00842
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 026355306
• PubMed: 25947919
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026355306
• Web Site: https://www.jstage.jst.go.jp/article/bpb/38/5/38_b14-00842/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016010362
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可