Surface Modification of Cell Scaffold in Aqueous Solution Using TiO₂Photocatalysis and Linker Protein L2 for Patterning Primary Neurons<i></i>

• Sekine Kohei

  School of Fundamental Science and Engineering, Waseda University

• Yamamoto Hideaki

  Frontier Research Institute for Interdisciplinary Sciences, Tohoku University Institute for Nanoscience and Nanotechnology, Waseda University

• Kono Sho

  School of Fundamental Science and Engineering, Waseda University

• Ikeda Takeshi

  Graduate School of Advanced Sciences and Matter, Hiroshima University

• Kuroda Akio

  Graduate School of Advanced Sciences and Matter, Hiroshima University

• Tanii Takashi

  School of Fundamental Science and Engineering, Waseda University Institute for Nanoscience and Nanotechnology, Waseda University

Bibliographic Information

Other Title

• Surface modification of cell scaffold in aqueous solution using tio2 photocatalysis and linker protein l2 for patterning primary neurons

Abstract

Titanium dioxide (TiO₂) photocatalysis can be applied to pattern proteins and cells under aqueous solution. In this work, we extended the application of this technique to patterning primary neurons, a type of cell with relatively weak adhesibility. For this purpose, we employed ribosomal protein L2 (RPL2) that has high affinity toward silica and metal oxides, including TiO₂, to stably bind a neuronal adhesion protein laminin to the TiO₂ surface. We utilized two types of molecular recognition to achieve this—binding of anti-laminin antibody to its antigen (laminin) and binding of protein A to the antibody. We show that a protein complex consisting of laminin/anti-laminin antibody/protein A-RPL2 is spontaneously formed by simply mixing the precursor proteins in solution phase. We then show that the surface coated with the protein complex supports stable growth of rat hippocampal neurons. Finally, we show that the cells can be selectively grown on the protein complex patterned with the TiO₂-assisted method. The protocol established in this work is a unique combination of a top-down micropatterning of the surface using TiO₂ photocatalysis and a bottom-up self-assembly of biomolecules, which can be further applied to pattern a wide range of proteins and cells. [DOI: 10.1380/ejssnt.2015.213]

Journal

• e-Journal of Surface Science and Nanotechnology

  e-Journal of Surface Science and Nanotechnology 13 (0), 213-218, 2015

  The Japan Society of Vacuum and Surface Science

Keywords

• Titanium oxide
• Catalysis
• Biological molecules - proteins
• Lithography
• Self-assembly
• Neuron
• Biointerface
• Surface engineering

Details

• CRID: 1390282680162219136
• NII Article ID: 130005068495
• DOI: 10.1380/ejssnt.2015.213
• ISSN: 13480391
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed