Selective extraction of nucleotides with fluorous biphasic system utilizing perfluoroalkylamine as ion-pair reagent

• HAYAMA Tadashi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• KIYOKAWA Ena

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YOSHIDA Hideyuki

  Faculty of Pharmaceutical Sciences, Fukuoka University

• IMAKYURE Osamu

  Faculty of Pharmaceutical Sciences, Fukuoka University

• YAMAGUCHI Masatoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

• NOHTA Hitoshi

  Faculty of Pharmaceutical Sciences, Fukuoka University

書誌事項

タイトル別名

• Selective Extraction of Nucleotides with Fluorous Biphasic System Utilizing Perfluoroalkylamine as an Ion-Pair Reagent

抄録

A novel and selective extraction method for nucleotides (AMP, ADP, ATP, GMP, GDP, and GTP) has been developed. In this study, we employed liquid-liquid based fluorous biphasic extraction system in the presence of a 4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,11-heptadecafluoroundecylamine (HFUA) as an ion-pair reagent. The ion-pairs between nucleotides and HFUA were selectively extracted to a mixture of tetradecafluorohexane and 1H,1H,2H,2H-tridecafluoro-1-n-octanol (3:1, v/v) as fluorous solvent via the affinity of perfluoroalkyl-containing compounds with each other, i.e. fluorous interaction. Then, the nucleotides in the fluorous solvent could be back-extracted with aqueous ammonia in acetonitrile as non-fluorous solvent, and the extracts were directly injected to LC system. A hydrophilic interaction chromatography was utilized for separation of the nucleotides by gradient elution with the mixture of acetonitrile,water, and ammonium carbonate buffer (pH 10.0) as mobile phase, and an ultraviolet detection at 254 nm was used. The extraction recoveries of nucleotides were in the range from 70.4 to 96.8%. This method could be applied to the determination of nucleotides in cell samples.

収録刊行物

• CHROMATOGRAPHY

  CHROMATOGRAPHY 36 (1), 13-18, 2015

  クロマトグラフィー科学会

キーワード

• Nucleotides
• Fluorous biphasic system
• Perfluoroalkylamine
• Ion-pair
• Cell sample

詳細情報

• CRID: 1390282680720841472
• NII論文ID: 130005069043
• NII書誌ID: AA1137755X
• DOI: 10.15583/jpchrom.2015.001
• ISSN: 13483315
• ISSN: 13428284
• NDL書誌ID: 026396048
• Web Site: http://id.ndl.go.jp/bib/026396048
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026396048
• 本文言語コード: en
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