高速原子間力顕微鏡による生体試料のダイナミクス観察  [in Japanese] Direct Observation of Dynamics of Biological Molecules with High–Speed AFM  [in Japanese]

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Author(s)

    • 内橋 貴之 Uchihashi Takayuki
    • 金沢大学 理工研究域 数物科学系 バイオAFM先端研究センター School of Mathematics and Physics/Bio–AFM Frontier Research Center, Kanazawa University

Abstract

Direct and real–time visualization of biological molecules at work is a straightforward and powerful approach to understanding the mechanisms of biomolecular processes. Recent advances of high–speed atomic force microscopy (HS–AFM) have opened new possibility to visualize dynamic events of label–free proteins under physiological conditions, at subsecond to sub–100 ms temporal and submolecular resolution. Here we overview the technical challenges to fast and low–invasive imaging of biomolecules with AFM. Then we illustrate some recent results demonstrating capability of the high–speed AFM for observing dynamic events of membrane related proteins such as conformational changes and two–dimensional crystal dynamics.

Journal

  • MEMBRANE

    MEMBRANE 39(5), 322-328, 2014

    THE MEMBRANE SOCIETY OF JAPAN

Codes

  • NII Article ID (NAID)
    130005069445
  • NII NACSIS-CAT ID (NCID)
    AN0023215X
  • Text Lang
    JPN
  • ISSN
    0385-1036
  • NDL Article ID
    025821119
  • NDL Call No.
    Z18-1127
  • Data Source
    NDL  J-STAGE 
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