Partitioning of Hydrophobic Molecules to Liposome Membranes Can Induce Variations in their Micro-Polarity and Micro-Viscosity

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Abstract

The micro-polarity and micro-viscosity of liposome membranes were evaluated to develop a platform for the localization of hydrophobic substrates in aqueous solution. The distribution ratios of benzaldoxime (BO) onto the zwitterionic 1,2-dioleoyl-<i>sn</i>-glycero-3-phosphocholine (DOPC) liposome and onto the cationic 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) liposome were almost the same, while those of benzonitrile oxide (BNO) onto DOTAP liposomes were higher than those of BO. Through the analysis of a polarity-sensitive 6-lauroyl-2-dimethylaminonaphthalene, the membrane surface of the DOTAP liposome was found to be dehydrated in the presence of substrates. Using a fluorescent probe 1,6-diphenyl-1,3,5-hexatriene, we found that the micro-viscosity of the DOTAP liposome membrane increased with BNO. These results indicate that the interaction of hydrophobic substrates induce variations in the microscopic membrane environment.

Journal

  • Solvent Extraction Research and Development, Japan

    Solvent Extraction Research and Development, Japan 22(1), 79-85, 2015

    Japan Association of Solvent Extraction

Codes

  • NII Article ID (NAID)
    130005071497
  • NII NACSIS-CAT ID (NCID)
    AA11114608
  • Text Lang
    ENG
  • ISSN
    1341-7215
  • NDL Article ID
    026425464
  • NDL Call No.
    Z63-C428
  • Data Source
    NDL  J-STAGE 
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