Partitioning of Hydrophobic Molecules to Liposome Membranes Can Induce Variations in their Micro-Polarity and Micro-Viscosity
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- IWASAKI Fumihiko
- Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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- SUGA Keishi
- Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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- KONDO Dai
- Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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- UMAKOSHI Hiroshi
- Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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Abstract
The micro-polarity and micro-viscosity of liposome membranes were evaluated to develop a platform for the localization of hydrophobic substrates in aqueous solution. The distribution ratios of benzaldoxime (BO) onto the zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposome and onto the cationic 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) liposome were almost the same, while those of benzonitrile oxide (BNO) onto DOTAP liposomes were higher than those of BO. Through the analysis of a polarity-sensitive 6-lauroyl-2-dimethylaminonaphthalene, the membrane surface of the DOTAP liposome was found to be dehydrated in the presence of substrates. Using a fluorescent probe 1,6-diphenyl-1,3,5-hexatriene, we found that the micro-viscosity of the DOTAP liposome membrane increased with BNO. These results indicate that the interaction of hydrophobic substrates induce variations in the microscopic membrane environment.
Journal
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- Solvent Extraction Research and Development, Japan
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Solvent Extraction Research and Development, Japan 22 (1), 79-85, 2015
Japan Association of Solvent Extraction
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Details 詳細情報について
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- CRID
- 1390282680719965056
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- NII Article ID
- 130005071497
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- NII Book ID
- AA11114608
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- ISSN
- 21884765
- 13417215
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- NDL BIB ID
- 026425464
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed