Fabrication of a Portable Electrochemiluminescence-induced Fluorescence Detection Chip with Microfluidic Excitation Source for Point-of-care Diagnostics
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- Tsuwaki Miho
- Waseda University
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- Kasahara Takashi
- Waseda University
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- Edura Tomohiko
- Kyushu University
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- Oshima Juro
- Kyushu University
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- Kunisawa Eri
- Kyushu University
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- Ishimatsu Ryoichi
- Kyushu University
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- Matsunami Shigeyuki
- Kyushu University
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- Imato Toshihiko
- Kyushu University
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- Adachi Chihaya
- Kyushu University
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- Shoji Shuichi
- Waseda University
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- Mizuno Jun
- Waseda University
Bibliographic Information
- Other Title
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- マイクロ流体電気化学発光素子を励起光源として用いたポータブル蛍光検出デバイスの作製
- マイクロ リュウタイ デンキ カガク ハッコウ ソシ オ レイキコウゲン ト シテ モチイタ ポータブル ケイコウ ケンシュツ デバイス ノ サクセイ
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Abstract
In this study, we propose an electrochemiluminescence (ECL)-induced fluorescence detection chip toward portable point-of-care diagnostics. A prototype chip consists of polydimethylsiloxane (PDMS)-based flow channels for target samples and on-demand multi-color microfluidic excitation sources. The microfluidic ECL cells were fabricated via a heterogeneous bonding technique through the use of self-assembled monolayers. The flow channels for target samples, which were formed by soft lithography, were combined with the excitation source. A pink fluorescence of resorufin was successfully detected with yellow ECL emission of 5,6,11,12-tetraphenyltetracene (rubrene). The proposed principle can open a new possibility for on-onsite multiple fluorescence detections.
Journal
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- IEEJ Transactions on Sensors and Micromachines
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IEEJ Transactions on Sensors and Micromachines 135 (6), 230-235, 2015
The Institute of Electrical Engineers of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204462885760
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- NII Article ID
- 130005072488
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- NII Book ID
- AN1052634X
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- ISSN
- 13475525
- 13418939
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- NDL BIB ID
- 026555567
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed
