Nifedipine Prevents Sodium Caprate-Induced Barrier Dysfunction in Human Epidermal Keratinocyte Cultures

• Uchino Yoshihiro

  Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University

• Matsumoto Junichi

  Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University

• Watanabe Takuya

  Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University

• Hamabashiri Masato

  Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University

• Tsuchiya Takashi

  Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University

• Kimura Ikuya

  Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University

• Yamauchi Atsushi

  Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University

• Kataoka Yasufumi

  Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University

Abstract

Tight junctions (TJs) of the epidermis play an important role in maintaining the epidermal barrier. TJ breakdown is associated with skin problems, such as wrinkles and transepidermal water loss (TEWL). Clinical studies have reported that topical nifedipine is effective in reducing the depth of wrinkles and improving TEWL. However, it remains unknown whether nifedipine influences the TJ function in the epidermis. In the present study, we investigated the effect of nifedipine on epidermal barrier dysfunction in normal human epidermal keratinocytes (NHEKs) treated with sodium caprate (C10), a TJ inhibitor. Nifedipine reversed the C10-decreased transepithelial electrical resistance values as a measure of disruption of the epidermal barrier. Immunocytochemical observations revealed that nifedipine improved the C10-induced irregular arrangement of claudin-1, a key protein in TJs. Taken together, these findings suggest that nifedipine prevents epidermal barrier dysfunction, at least in part, by reconstituting the irregular claudin-1 localization at TJs in C10-treated NHEKs.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 38 (6), 926-929, 2015

  The Pharmaceutical Society of Japan

Keywords

• epidermal barrier function
• nifedipine
• sodium caprate
• transepithelial electrical resistance
• normal human epidermal keratinocyte

Details

• CRID: 1390001204634034944
• NII Article ID: 130005072663
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.b14-00854
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 026408472
• PubMed: 26027835
• Web Site: http://id.ndl.go.jp/bib/026408472
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026408472
• Web Site: https://www.jstage.jst.go.jp/article/bpb/38/6/38_b14-00854/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016026456
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed