Thermal-induced Immuno-nephelometry Using Gold Nanoparticles Conjugated with a Thermoresponsive Polymer for the Detection of Avidin

• UEHARA Nobuo

  Department of Applied Chemistry, Graduate School of Engineering, Utsunomiya University

• NUMANAMI Yoshikuni

  Department of Applied Chemistry, Graduate School of Engineering, Utsunomiya University

• OBA Toru

  Department of Applied Chemistry, Graduate School of Engineering, Utsunomiya University

• ONISHI Noriyuki

  JNC Petrochemical Corporation, Goi Research Center

• XIE Xiaomao

  JNC Petrochemical Corporation, Goi Research Center

Abstract

Thermoresponsive immunonephelometry was achieved with biotinylated poly(acrylate) and thermoresponsive gold nanocomposites composed of 13-nm gold nanoparticles and thermoresponsive polymers containing triethylenetetramine and biotin groups. The avidin–biotin interaction was used to model an immunoreaction in order to demonstrate thermoresponsive immunonephelometry. In the absence of avidin, positively charged gold nanocomposites electrostatically interacted with biotinylated poly(acrylate) to form binary complexes, in which the charges canceled each other out. The charge cancelation resulted in the binary complexes precipitating when the solution was heated above the phase-transition temperature. However, adding avidin formed ternary sandwich complexes through the avidin–biotin interaction. The ternary complexes remained sufficiently soluble above the phase-transition temperature because of the spatial isolation of the positive and negative charges. The transmittance of the solution containing the thermoresponsive gold nanocomposites and biotinylated poly(acrylate) at 37°C increased as the avidin concentration increased. A sigmoidal profile was observed from 10^−6.5 to 10^−5.5 mol/L. The concentration of avidin spiked in bovine serum was determined by our method.

Journal

• Analytical Sciences

  Analytical Sciences 31 (6), 495-501, 2015

  The Japan Society for Analytical Chemistry

Keywords

• Thermoresponsive polymer
• gold nanoparticles
• immunonephelometry
• avidin

Details

• CRID: 1390282679235706496
• NII Article ID: 130005075717
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.31.495
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 026440973
• PubMed: 26063011
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026440973
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.495.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.495/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/6/31_495/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed