A New Fractionation and Recovery Method of Viral Genomes Based on Nucleic Acid Composition and Structure Using Tandem Column Chromatography

• Urayama Syun-ichi

  Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

• Yoshida-Takashima Yukari

  Department of Subsurface Geobiological Analysis and Research, JAMSTEC

• Yoshida Mitsuhiro

  Department of Subsurface Geobiological Analysis and Research, JAMSTEC

• Tomaru Yuji

  National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency

• Moriyama Hiromitsu

  Laboratory of Molecular and Cellular Biology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology

• Takai Ken

  Department of Subsurface Geobiological Analysis and Research, JAMSTEC

• Nunoura Takuro

  Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Abstract

Metagenomic studies have revealed the unexplored diversity of the environmental virosphere. However, most studies are biased towards specific types of viral genomes due to the absence of universal methods to access all viral genome types. In the present study, we established a novel system to efficiently separate single- and double-stranded DNA/RNA viral genomes using hydroxyapatite and cellulose chromatography. This method will allow us to quantitatively and simultaneously access four types of viral genomes and will provide important clues to further understand previously unexplored environmental viral populations and obtain potentially unbiased libraries from environmental viral communities.

Journal

• Microbes and Environments

  Microbes and Environments 30 (2), 199-203, 2015

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• viral ecology
• viral genome
• column chromatography
• nucleic acids

Details

• CRID: 1390282679323942144
• NII Article ID: 130005077995
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me14174
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 026498661
• PubMed: 26004793
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026498661
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed