Depolarization-induced Intracellular Free Calcium Concentration Increases Show No Desensitizing Effect in Rat Odontoblasts

• Kojima Yuki

  Department of Physiology, Tokyo Dental College

• Higashikawa Asuka

  Department of Physiology, Tokyo Dental College

• Kimura Maki

  Department of Physiology, Tokyo Dental College

• Sato Masaki

  Department of Physiology, Tokyo Dental College

• Mochizuki Hiroyuki

  Department of Physiology, Tokyo Dental College

• Ogura Kazuhiro

  Department of Physiology, Tokyo Dental College

• Sase Toshiyuki

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Shinya Akinori

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Kobune Kunio

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Furuya Tadashi

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Sato Toru

  Department of Crown and Bridge Prosthodontics, Tokyo Dental College

• Shibukawa Yoshiyuki

  Department of Physiology, Tokyo Dental College

• Tazaki Masakazu

  Department of Physiology, Tokyo Dental College

抄録

Odontoblasts play an important role in the transduction of the sensory signals underlying dentinal pain. Transmembrane voltage-independent Ca²⁺ influx in odontoblasts has been well described. Voltage-dependent Ca²⁺ influx has also been reported, but its biophysical properties remain unclear. The aim of the present study was to investigate the desensitizing effect of voltage-dependent Ca²⁺ influx in rat odontoblasts by measuring depolarization-induced intracellular free Ca²⁺ concentrations ([Ca²⁺ ]_i ). Odontoblasts on dental pulp slices from newborn rats were acutely isolated and [Ca²⁺ ]_i measured by using fura-2 fluorescence. Repeated application of extracellular high-K⁺ solution (50 mM), which induces membrane depolarization-elicited repeated and transient increases in [Ca²⁺ ]_i in the presence of extracellular Ca²⁺. Increases in depolarization-induced [Ca²⁺ ]_i showed no significant desensitizing effect (p >0.05; Friedman test). These results suggest that odontoblasts express a voltage-dependent Ca²⁺ influx pathway with no desensitizing properties.

収録刊行物

• The Bulletin of Tokyo Dental College

  The Bulletin of Tokyo Dental College 56 (2), 131-134, 2015

  東京歯科大学

キーワード

• Odontoblasts
• Depolarization
• Dentinal pain
• Voltage-dependent Ca²⁺ influx

詳細情報

• CRID: 1390282679563889280
• NII論文ID: 130005078126
• NII書誌ID: AA00592969
• DOI: 10.2209/tdcpublication.56.131
• ISSN: 00408891
• PubMed: 26085001
• Web Site: https://search.jamas.or.jp/link/ui/2016127995
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可