Diallyl disulfide administration increases the number of B-lymphocytes in the rat spleen

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著者

    • Hashizume Yoko
    • Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University
    • Shirato Ken
    • Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University
    • Imaizumi Kazuhiko
    • Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University|Global Center of Excellence Doctoral Program, Graduate School of Sport Sciences, Waseda University

抄録

Diallyl disulfide (DADS), the major sulfur compound in garlic, reduces the number of circulating T-lymphocytes, B-lymphocytes, and monocytes via activation of the hypothalamus-pituitary-adrenal axis. However, the translocation of those cells that migrate in response to DADS administration is still unclear. Therefore, in this study, we examined the effects of DADS administration on a number of lymphocyte subsets and monocyte-derived cells including macrophages (monocytes/macrophages) in spleen, the largest secondary lymphoid organ. Ten-wk-old male Sprague-Dawley rats were orally administered with DADS (dose = 20 mg/kg body weight) or equivalent volume of vehicle. The spleen was harvested 4 hr after administration, and then the splenic cells were isolated and the total number of cells was counted. T-lymphocytes, B-lymphocytes, natural killer (NK) cells, and monocytes/macrophages were fractionated by flow-cytometry and the total number of these cells was calculated. The total number of splenic cells was significantly increased by 1.18-fold after DADS administration. Among the lymphocyte subsets in the spleen, the number of B-lymphocytes significantly increased by 1.28-fold after DADS administration. The number of T-lymphocytes also showed a tendency to increase. However, the number of NK cells and monocytes/macrophages did not change after DADS administration. These results suggest that B-lymphocytes migrate from the circulation and translocate to the spleen in response to DADS administration.

収録刊行物

  • Fundamental Toxicological Sciences

    Fundamental Toxicological Sciences 1(4), 115-121, 2014

    一般社団法人 日本毒性学会

各種コード

  • NII論文ID(NAID)
    130005083351
  • 本文言語コード
    ENG
  • データ提供元
    J-STAGE 
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