Age-Dependent Decrease of DNA Hydroxymethylation in Human T Cells
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Hydroxymethylcytosine (hmC) is a natural nucleobase, which is converted from methylcytosine (mC) by tet methylcytosine dioxygenase (TET) family (TET1-3) enzymes. Decrease of genomic hmC is postulated to confer a risk for myeloid-lineage as well as T-cell neoplasms, based on the fact that loss-of-function mutations in the <i>TET2</i> gene were frequently identified in these diseases. The relationship between hmC and aging remains to be elucidated. Here, we demonstrated that hmC content decreased with age in the peripheral blood T cells of 53 human volunteers. We further identified that the mRNA expression levels of <i>TET1</i> and <i>TET3</i> decreased with age, while those of <i>TET2</i> were not influenced by age. The genomic hmC content was correlated with the mRNA expression level of <i>TET3</i>, but not those of <i>TET1</i> and <i>TET2</i>. Our study suggests the presence of new epigenetic regulatory mechanisms in aging T cells.
- Journal of Clinical and Experimental Hematopathology
Journal of Clinical and Experimental Hematopathology 55(1), 1-6, 2015
The Japanese Society for Lymphoreticular Tissue Research