An upstream open reading frame represses expression of a tomato homologue of Arabidopsis <i>ANAC096</i>, a NAC domain transcription factor gene, in a peptide sequence-dependent manner

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Many eukaryotic mRNAs contain one or more upstream open reading frames (uORFs) in their 5′ untranslated regions (5′-UTRs). Some uORFs encode regulatory peptides that repress translation of the main ORF. To comprehensively identify uORFs encoding regulatory peptides, genome-wide searches for uORFs with evolutionarily conserved amino acid sequences, referred to as conserved peptide uORFs (CPuORFs), have been conducted using bioinfomatic approaches. To date, more than 40 homology groups of CPuORFs have been identified in dicotyledonous plants. The <i>Arabidopsis thaliana</i> <i>ANAC096</i> gene is one of the CPuORF-containing genes; however, the <i>ANAC096</i> CPuORF exerts only little peptide sequence-dependent effect on expression of the main ORF. Here, we investigated the effect of the CPuORF sequence of a tomato <i>ANAC096</i> homologue on expression of the main ORF, because it has a more highly conserved amino acid sequence than the <i>ANAC096</i> CPuORF. Mutational analyses revealed that the CPuORF of the tomato <i>ANAC096</i> homologue represses main ORF expression in a peptide sequence-dependent manner, and determined the critical amino acid residues of the CPuORF peptide responsible for the repression. This study identified a novel peptide sequence-dependent regulatory uORF and demonstrated that the level of uORF peptide-mediated repression can differ among closely related homologues.


  • Plant Biotechnology

    Plant Biotechnology 32(2), 157-163, 2015

    Japanese Society for Plant Cell and Molecular Biology


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