The inhibitory effect of paclitaxel on (KV2.1) K^+ current in H9c2 cells

• KITAMURA NAOKO

  Department of Pharmacology, Fukushima Medical University, School of Medicine

• SAKAMOTO KAZUHO

  Department of Pharmacology, Fukushima Medical University, School of Medicine

• ONO TOMOYUKI

  Department of Pharmacology, Fukushima Medical University, School of Medicine

• KIMURA JUNKO

  Department of Pharmacology, Fukushima Medical University, School of Medicine

書誌事項

タイトル別名

• THE INHIBITORY EFFECT OF PACLITAXEL ON (KV2.1) K⁺ CURRENT IN H9c2 CELLS

抄録

Using the whole-cell voltage clamp technique, we investigated the effect of paclitaxel, an anticancer agent which promotes microtubule formation, on K⁺ current in H9c2 cells originated from rat embryonic cardiac myocytes. Paclitaxel inhibited Kv2.1 voltage-dependent K⁺ current (IKur) with ultra-rapidly activating and slowly inactivating kinetics in a concentration-dependent manner. The inhibitory effect of paclitaxel on IKur was time-dependent and more marked at 200 ms after the onset than at the beginning of the depolarizing pulse. The IC₅₀ value of paclitaxel was 1.1 µM at 200 ms. The time-dependent inhibition suggests that paclitaxel might be an open channel blocker of Kv2.1. This inhibition of Kv2.1 may be involved in the adverse effects of paclitaxel on cardiac and neuronal cells.

収録刊行物

• 福島医学会

  福島医学会 61 (1), 47-53, 2015

  福島医学会

キーワード

• paclitaxel
• Kv2.1
• K⁺current
• H9c2 cells
• whole-cell clamp

詳細情報

• CRID: 1390001206306399872
• NII論文ID: 130005087004
• NII書誌ID: AA0065246X
• DOI: 10.5387/fms.2014-34
• ISSN: 21854610
• ISSN: 00162590
• PubMed: 25994081
• Web Site: http://ir.fmu.ac.jp/dspace/handle/123456789/461
• Web Site: https://search.jamas.or.jp/link/ui/2016216419
• 本文言語コード: en
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  • IRDB
  • Crossref
  • PubMed
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  • KAKEN
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