<b>Responses of infected dental pulp to αTCP-containing antimicrobials in rat molars</b>

DOI 1 Citations 37 References Open Access
  • Sato Takuichi
    Division of Oral Ecology and Biochemistry, Department of Oral Biology, Tohoku University Graduate School of Dentistry
  • Kenmotsu Shin-ichi
    Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences
  • Nakakura-Ohshima Kuniko
    Pediatric Dentistry, Niigata University Medical and Dental Hospital
  • Takahashi Nobuhiro
    Division of Oral Ecology and Biochemistry, Department of Oral Biology, Tohoku University Graduate School of Dentistry
  • Ohshima Hayato
    Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences

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Abstract

α-tricalcium phosphate (αTCP) with the addition of antimicrobials such as ciprofloxacin, metronidazole, and cefaclor (3Mix) has been applied to sterilize the infected dentin and pulp in vivo. Both clinical and animal experiments have shown that 3Mix is effective for sterilizing infected tissues. However, the responses of the infected dental pulp to 3Mix remain to be fully determined at the cellular level. This study aims to clarify the responses of neural elements and immune cells to antimicrobials during the healing process of infected pulp using immunohistochemistry for protein gene product (PGP) 9.5 and class II major histocompatibility complex molecules using both light and electron microscopy. An artificial pulp exposure was prepared on the maxillary molar of 14-week-old rats and maintained without any treatment for 12 - 24 h. Subsequently, the exposed pulp was covered with αTCP or αTCP containing 3Mix, followed with glass ionomer cement. A pulp abscess lacking both dendritic cells and PGP 9.5-reactive nerve fibers was induced after pulp capping with αTCP; in contrast, numerous dendritic cells accumulated along the pulp-dentin border followed by the differentiation of odontoblast-like cells and matrix deposition after the application of αTCP containing 3Mix. PGP 9.5-reactive nerve fibers were also densely distributed and surrounded the accumulated dendritic cells in the medial dental pulp beneath αTCP containing 3Mix. The findings indicate that the application of αTCP containing 3Mix to the infected pulp induces an intense accumulation of dendritic cells, suggesting that these cells play crucial roles in the differentiation of odontoblast-like cells under pathological conditions.

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