Leaf margin phenotype-specific restriction-site-associated DNA-derived markers for pineapple (Ananas comosus L.)

• Urasaki Naoya

  Okinawa Prefectural Agricultural Research Center

• Goeku Satoko

  Okinawa Prefectural Agricultural Research Center

• Kaneshima Risa

  Okinawa Prefectural Agricultural Research Center

• Takamine Tomonori

  Okinawa Prefectural Institute of Health and Environment

• Tarora Kazuhiko

  Okinawa Prefectural Agricultural Research Center

• Takeuchi Makoto

  Okinawa Prefectural Agricultural Research Center, Nago Branch

• Moromizato Chie

  Okinawa Prefectural Agricultural Research Center, Nago Branch

• Yonamine Kaname

  Okinawa Prefectural Agricultural Research Center, Nago Branch

• Hosaka Fumiko

  NARO Institute of Fruit Tree Science

• Terakami Shingo

  NARO Institute of Fruit Tree Science

• Matsumura Hideo

  Gene Research Center, Shinshu University

• Yamamoto Toshiya

  NARO Institute of Fruit Tree Science

• Shoda Moriyuki

  Okinawa Prefectural Agricultural Research Center, Nago Branch

書誌事項

タイトル別名

• Leaf margin phenotype-specific restriction-site-associated DNA-derived markers for pineapple (<i>Ananas comosus</i> L.)

抄録

To explore genome-wide DNA polymorphisms and identify DNA markers for leaf margin phenotypes, a restriction-site-associated DNA sequencing analysis was employed to analyze three bulked DNAs of F1 progeny from a cross between a ‘piping-leaf-type’ cultivar, ‘Yugafu’, and a ‘spiny-tip-leaf-type’ variety, ‘Yonekura’. The parents were both Ananas comosus var. comosus. From the analysis, piping-leaf and spiny-tip-leaf gene-specific restriction-site-associated DNA sequencing tags were obtained and designated as PLSTs and STLSTs, respectively. The five PLSTs and two STSLTs were successfully converted to cleaved amplified polymorphic sequence (CAPS) or simple sequence repeat (SSR) markers using the sequence differences between alleles. Based on the genotyping of the F1 with two SSR and three CAPS markers, the five PLST markers were mapped in the vicinity of the P locus, with the closest marker, PLST1_SSR, being located 1.5 cM from the P locus. The two CAPS markers from STLST1 and STLST3 perfectly assessed the ‘spiny-leaf type’ as homozygotes of the recessive s allele of the S gene. The recombination value between the S locus and STLST loci was 2.4, and STLSTs were located 2.2 cM from the S locus. SSR and CAPS markers are applicable to marker-assisted selection of leaf margin phenotypes in pineapple breeding.

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 65 (3), 276-284, 2015

  日本育種学会

キーワード

• CAPS
• leaf margin phenotype
• pineapple (<i>Ananas comosus</i>)
• RAD-seq
• SSR

詳細情報

• CRID: 1390282679700595328
• NII論文ID: 130005088164
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.65.276
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 026560441
• Web Site: http://id.ndl.go.jp/bib/026560441
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026560441
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/65/3/65_276/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
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