DNA structures under molecular crowding conditions with a phosphorylcholine derivative (MPC)  [in Japanese]

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Author(s)

    • Ueda Yu-mi
    • Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University
    • Konno Tomohiro
    • Department of Bioengineering, School of Engineering, The University of Tokyo
    • Ishihara Kazuhiko
    • Department of Bioengineering, School of Engineering, The University of Tokyo|Department of Materials Engineering, School of Engineering, The University of Tokyo
    • Sugimoto Naoki
    • Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University|Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University
    • Miyoshi Daisuke
    • Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University|Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University

Abstract

The molecular environment of a living cell is totally different from that of a typical test tube solution; about 30% of the volume of the cell interior is occupied by large biomolecules, small metabolites, and osmolytes, leading to molecular crowding conditions. In particular, there are large amounts of zwitterionic molecules that act as osmolytes, and amino acids. Here, we studied the thermodynamics of the canonical DNA duplex and non-canonical DNA G-quadruplex structures under molecular crowding conditions using a phosphorylcholine derivative, 2-methacryloyloxyethyl phosphorylcholine (MPC), as a model compound of a naturally occurring zwitterionic molecule. It was found that MPC stabilized the DNA G-quadruplex structure, whereas it destabilized the DNA duplex.

Journal

  • Transactions of the Materials Research Society of Japan

    Transactions of the Materials Research Society of Japan 40(2), 99-102, 2015

    The Materials Research Society of Japan

Codes

  • NII Article ID (NAID)
    130005089522
  • Text Lang
    JPN
  • ISSN
    1382-3469
  • Data Source
    J-STAGE 
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