<i>Porphyromonas bronchialis</i> sp. nov. Isolated from Intraoperative Bronchial Fluids of a Patient with Non-Small Cell Lung Cancer

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Author(s)

    • Sato Takuichi
    • Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry
    • Tanaka Kaori
    • Division of Anaerobic Research, Life Science Research Center, Gifu University
    • Takahashi Nobuhiro
    • Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry
    • Tomida Junko
    • Department of Microbiology, School of Pharmacy, Aichi Gakuin University
    • Fujiwara Nagatoshi
    • Department of Food and Nutrition, Faculty of Contemporary Human Life Science, Tezukayama University
    • Hasegawa Ayako
    • Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry|Department of Microbiology, School of Pharmacy, Aichi Gakuin University
    • Hoshikawa Yasushi
    • Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University
    • Matsuyama Junko
    • Division of Pediatric Dentistry, Niigata University Graduate School of Medical and Dental Sciences
    • Ishida Naoko
    • Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry
    • Kondo Takashi
    • Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University

Abstract

<i>Porphyromonas</i> strains, including <i>Porphyromonas</i>-like strains, have been isolated from oral and various other systemic infections. The characterization of such strains is a crucial issue, because such information contributes to both the taxonomy of anaerobic bacteria and the clinical aspects of infectious diseases. We previously isolated four <i>Porphyromonas</i>-like strains from intraoperative bronchial fluids of a patient with non-small cell lung cancer. This study aimed to characterize the genetic, biochemical and chemotaxonomic aspects of these isolates. Each strain only grew under anaerobic conditions and their colony morphology was convex, 0.1-1.0 mm in diameter, light gray, and slightly glistening colony, with no black or brown pigmentation on blood agar plates after five-day incubation. The pigmentation was helpful to differentiate the isolates from other <i>Porphyromonas</i>, as most of <i>Porphyromonas</i> species show the pigmentation. In the 16S rRNA gene phylogenetic analysis (98% sequence identity of isolates indicates the same species), the four isolates were closely related to one another (99.7-100.0%), but not related to <i>Porphyromonas </i>(<i>P.</i>)<i> catoniae</i>, the closest species (96.9%). In addition, the DNA-DNA hybridization data revealed less than 16% similarity values between a representative isolate and the<i> P. catoniae</i>, indicating that the strains were genetically independent. Biochemically, the isolates could be differentiated from closely related species, i.e., <i>P. catoniae</i>,<i> P. gingivalis</i>,<i> P. gulae</i>, and <i>P. pogonae</i>, with trypsin activity (negative only in the isolates) and leucine arylamidase activity (positive only in the isolates). We therefore propose a new species to include these isolates: <i>Porphyromonas bronchialis</i> sp. nov.

Journal

  • The Tohoku Journal of Experimental Medicine

    The Tohoku Journal of Experimental Medicine 237(1), 31-37, 2015

    Tohoku University Medical Press

Codes

  • NII Article ID (NAID)
    130005094770
  • Text Lang
    ENG
  • ISSN
    0040-8727
  • Data Source
    J-STAGE 
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