Effect of a novel fluorapatite-forming calcium phosphate cement with calcium silicate on osteoblasts in comparison with mineral trioxide aggregate
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- Suzuki Yusuke
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- Hayashi Makoto
- Department of Endodontics, Nihon University School of Dentistry
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- Tanabe Natsuko
- Department of Biochemistry, Nihon University School of Dentistry
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- Yasukawa Takuya
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- Hirano Yoriyuki
- Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
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- Takagi Shozo
- American Dental Association Foundation, Dr. Anthony Volpe Research Center, National Institute of Standards and Technology
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- C. Chow Laurence
- American Dental Association Foundation, Dr. Anthony Volpe Research Center, National Institute of Standards and Technology
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- Suzuki Naoto
- Department of Biochemistry, Nihon University School of Dentistry
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- Ogiso Bunnai
- Department of Endodontics, Nihon University School of Dentistry
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We compared the effects of treatment with fluorapatite-forming calcium phosphate cement (FA-forming CPC) containing tricalcium silicate (TCS) and those of mineral trioxide aggregate (MTA), the gold standard endodontic cement, on cultured osteoblast-like cells (ROS 17/2.8 cells; ROS cells). The FA-forming CPC powder consisted of 61.29% CaHPO4, 32.26% CaCO3, and 6.45% NaF. One part TCS was combined with nine parts FA-forming CPC powder to make FA-forming CPC with TCS. A 1.5-M phosphate solution was mixed as a cement liquid with a powder/liquid ratio of 2.22. Cell culture was carried out using cell culture inserts, whereby each test material was put on a porous membrane insert in the cell culture plate. Proliferation, morphologic changes, and alkaline phosphatase activity in ROS cells were measured in the presence of FA-forming CPC with TCS and MTA and compared. The logarithmic growth phase and cellular morphologic changes in ROS cells were identical in all experimental groups. Additionally, no significant difference in alkaline phosphatase activity was noted in ROS cells exposed to FA-forming CPC with TCS and those exposed to MTA. In conclusion, FA-forming CPC with TCS has characteristics identical to those of MTA under the present experimental conditions and may thus be useful for endodontic applications. (J Oral Sci 57, 25-30, 2015)
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
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Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 57 (1), 25-30, 2015
日本大学歯学部
