Repression of the activities of two extracytoplasmic function <b>σ</b> factors, <b>σ</b>^M and <b>σ</b>^V, of <i>Bacillus subtilis</i> by glucolipids in <i>Escherichia coli</i> cells

• Seki Takahiro

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• Mineshima Ryota

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• Hashimoto Michihiro

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• Matsumoto Kouji

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• Hara Hiroshi

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

• Matsuoka Satoshi

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University

Bibliographic Information

Other Title

• Repression of the activities of two extracytoplasmic function σ factors, σM and σV, of Bacillus subtilis by glucolipids in Escherichia coli cells
• Repression of the activities of extracytoplasmic function σ factors, σM and σV of Bacillus subtilis by glucolipids in Escherichia coli cells.

Abstract

Extracytoplasmic function (ECF) σ factors respond to environmental stresses and regulate numerous genes required for adaptation. Under normal growth conditions, the ECF σ factors are sequestered by transmembrane anti-σ factor proteins, from which they are released under stress conditions. In Bacillus subtilis ugtP null mutant cells, which lack glucolipids, three of the seven ECF σ factors, σ^M, σ^V and σ^X, are activated. The Escherichia coli cell membrane does not contain glucolipids. When the genes for these three ECF σ and anti-σ factors were introduced into E. coli cells, expression of lacZ fused to the ECF σ factor-regulated promoters indicated ECF σ factor activity. Additional expression of the ugtP gene in these E. coli cells led to the synthesis of small amounts of glucolipids, and the activities of σ^M and σ^V were repressed, but the activity of σ^X was unaffected. It is likely that glucolipids directly influence anti-σ^M and anti-σ^V factors by stabilizing conformations that sequester the respective ECF σ factors.

Journal

• Genes & Genetic Systems

  Genes & Genetic Systems 90 (2), 109-114, 2015

  The Genetics Society of Japan

Keywords

• anti-σ factor
• <i>Bacillus subtilis</i>
• ECF σ factor
• <i>Escherichia coli</i>
• glucolipid

Details

• CRID: 1390001205474220160
• NII Article ID: 130005098892
• NII Book ID: AA11077421
• DOI: 10.1266/ggs.90.109
• ISSN: 18805779
• ISSN: 13417568
• NDL BIB ID: 026733489
• PubMed: 26399770
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026733489
• Web Site: https://www.jstage.jst.go.jp/article/ggs/90/2/90_109/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed