Loop-Mediated Isothermal Amplification (LAMP) for Detection of <i>Campylobacter jejuni</i> and <i>C. coli</i> in Thai Children with Diarrhea
-
- Pham Ngan Thi Kim
- Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
-
- Trinh Quang Duy
- Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
-
- Khamrin Pattara
- Department of Microbiology, Faculty of Medicine, Chiang Mai University
-
- Ukarapol Nuthapong
- Department of Pediatrics, Faculty of Medicine, Chiang Mai University
-
- Kongsricharoern Tipachan
- Pediatric Hematology Unit, Nakornping Hospital
-
- Yamazaki Wataru
- Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki
-
- Komine-Aizawa Shihoko
- Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
-
- Okitsu Shoko
- Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
-
- Maneekarn Niwat
- Department of Microbiology, Faculty of Medicine, Chiang Mai University
-
- Hayakawa Satoshi
- Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
-
- Ushijima Hiroshi
- Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine
Search this article
Abstract
Campylobacter species are common causes of bacterial diarrhea, and Campylobacter jejuni and C. coli are known as the predominant causative agents in humans. Recent studies suggested that loop-mediated isothermal amplification (LAMP) is an efficient and practical tool for rapid detection of C. jejuni and C. coli in clinical samples. We used LAMP to screen 151 stool samples for Campylobacter; these samples were collected in 2012 from Thai children with diarrhea. The PCR method discriminated C. jejuni and C. coli among the detected Campylobacter strains; these species were subjected to sequencing of the hipO gene (in C. jejuni) or the ask gene (in C. coli). The results suggest that the prevalence of Campylobacter infection among Thai children with diarrhea is 8.6%, and C. jejuni is the most prevalent species.
Journal
-
- Japanese Journal of Infectious Diseases
-
Japanese Journal of Infectious Diseases 68 (5), 432-433, 2015
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee