A Molecular and Serological Survey of <i>Rickettsiales</i> Bacteria in Wild Sika Deer (<i>Cervus nippon nippon</i>) in Shizuoka Prefecture, Japan: High Prevalence of <i>Anaplasma</i> Species

• Wu Dongxing

  Laboratory of Microbiology, Department of Food and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka

• Wuritu

  Laboratory of Microbiology, Department of Food and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka State Nationalities Affairs Commission and Department of Educational Key Lab of Minority Traditional Medicine, MINZU University of China

• Yoshikawa Yuko

  Laboratory of Microbiology, Department of Food and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka

• Gaowa

  Laboratory of Microbiology, Department of Food and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka

• Kawamori Fumihiko

  Department of Microbiology, Shizuoka Institute of Environmental and Hygiene

• Ikegaya Asaka

  Department of Microbiology, Shizuoka Institute of Environmental and Hygiene

• Ohtake Masayoshi

  Shizuoka Prefectural Research Institute of Agriculture and Forestry

• Ohashi Masataka

  Shizuoka Prefectural Research Institute of Agriculture and Forestry

• Shimada Masahiko

  Laboratory of Microbiology, Department of Food and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka

• Takada Ayumi

  Laboratory of Microbiology, Department of Food and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka

• Iwai Katsuki

  Laboratory of Microbiology, Department of Food and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka

• Ohashi Norio

  Laboratory of Microbiology, Department of Food and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka

Bibliographic Information

Other Title

• A Molecular and Serological Survey of Rickettsiales Bacteria in Wild Sika Deer (Cervus nippon nippon) in Shizuoka Prefecture, Japan : High Prevalence of Anaplasma Species
• Molecular and serological survey of Rickettsiales bacteria in wild sika deer (Cervus nippon nippon) in Shizuoka prefecture, Japan: high prevalence of Anaplasma species

Abstract

We surveyed Rickettsiales bacteria, including Rickettsia, Ehrlichia, Anaplasma, and Neoehrlichia, in wild sika deer (Cervus nippon nippon) from Shizuoka prefecture, Japan. In spleen samples from 187 deer, Anaplasma phagocytophilum (deer type), A. bovis, and A. centrale were successfully detected by PCR assay targeting to 16S rDNA or p44/msp2, and their positive rates were 96.3% (180/187), 53.5% (100/187), and 78.1% (146/187), respectively. Additionally, 2 or 3 Anaplasma species could be detected from a single deer in 165 spleen samples (88.2%), indicating dual or triple infection. In contrast, A. phagocytophilum (human type) 16S rDNA, Rickettsia gltA, Ehrlichia p28/omp-1, and Neoehrlichia 16S rDNA could not be amplified. The serological test of 105 deer serum samples by immunofluorescence assay showed that the detection of antibodies against antigens of A. phagocytophilum HZ (US-human isolate) and Rickettsia japonica YH were 29.5% (31/105) and 75.2% (79/105), respectively. These findings suggest that A. phagocytophilum (deer type), A. centrale, and A. bovis are highly dominant and prevalent in wild sika deer from Shizuoka, a central region of Japan, and that the antibodies against some Rickettsiales bacteria have also been retained in deer blood.

Journal

• Japanese Journal of Infectious Diseases

  Japanese Journal of Infectious Diseases 68 (5), 434-437, 2015

  National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

Keywords

• <i>Rickettsiales</i> bacteria
• deer
• <i>Anaplasma</i>

Details

• CRID: 1390282681217480960
• NII Article ID: 130005098926
• NII Book ID: AA1132885X
• DOI: 10.7883/yoken.jjid.2015.003
• ISSN: 18842836
• ISSN: 13446304
• NDL BIB ID: 026764371
• PubMed: 25971318
• Web Site: http://id.ndl.go.jp/bib/026764371
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026764371
• Web Site: https://www.jstage.jst.go.jp/article/yoken/68/5/68_JJID.2015.003/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016248587
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed