Effects of Hemagglutination Activity in the Serum of a Deep-Sea Vent Endemic Crab, <i>Shinkaia Crosnieri</i>, on Non-Symbiotic and Symbiotic Bacteria

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Author(s)

    • WATSUJI TOMOO [他] Nakagawa Satoshi
    • Laboratory of Marine Environmental Microbiology, Graduate School of Agriculture, Kyoto University|Subsurface Geobiology and Advanced Research project (SUGAR), Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
    • Tateno Hiroaki
    • Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST)
    • Watsuji Tomoo
    • Subsurface Geobiology and Advanced Research project (SUGAR), Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
    • Mino Sayaka
    • Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University
    • Sawabe Tomoo
    • Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University
    • Takai Ken
    • Subsurface Geobiology and Advanced Research project (SUGAR), Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Abstract

In deep-sea hydrothermal environments, most invertebrates associate with dense populations of symbiotic microorganisms in order to obtain nutrition. The molecular interactions between deep-sea animals and environmental microbes, including their symbionts, have not yet been elucidated in detail. Hemagglutinins/lectins, which are carbohydrate-binding proteins, have recently been reported to play important roles in a wide array of biological processes, including the recognition and control of non-self materials. We herein assessed hemagglutination activity in the serum of a deep-sea vent endemic crab, <i>Shinkaia crosnieri</i>, which harbors chemosynthetic epibionts on its plumose setae. Horse and rabbit erythrocytes were agglutinated using this serum (opt. pH 7.5 and opt. temperature 15°C). Agglutinating activity was inhibited by eight kinds of sugars and several divalent cations, did not require any divalent metal ions, and remained detectable even after heating the serum at 100°C for 30 min. By using fluorescently labeled serum, we demonstrated that deep-sea crab serum components bound to the epibionts even in the presence of sugars. This study represents the first immunological assessment of a deep-sea vent endemic crab and demonstrated the possibility of a non-lectin-mediated symbiont-host interaction.

Journal

  • Microbes and Environments

    Microbes and Environments 30(3), 228-234, 2015

    Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology

Codes

  • NII Article ID (NAID)
    130005099200
  • NII NACSIS-CAT ID (NCID)
    AA11551577
  • Text Lang
    ENG
  • ISSN
    1342-6311
  • NDL Article ID
    026766271
  • NDL Call No.
    Z54-J644
  • Data Source
    NDL  J-STAGE 
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