Chemoenzymatic Synthesis of Glycoprotein Using the Peptide Ligation Chemistry

• Asahina Yuya

  Institute for Protein Research, Osaka University

Abstract

The chemoenzymatic method using glycosynthase is one of potent strategies for the synthesis of glycoproteins having a homogeneous glycan. In this method, the efficient preparation of the N-acetylglucosaminylated protein is of great importance. Thus, for producing this compound with a high efficiency, we developed the sequential thioester method and the fast preparation method of the N-acetylglucosaminyl peptide. These methods realized the efficient preparation of glycoproteins having GlcNAc. Finally, homogeneous glycoproteins, the Tim-3 Ig domain and emmprin were successfully synthesized by the transglycosylation using glycosynthase.

Journal

• Trends in Glycoscience and Glycotechnology

  Trends in Glycoscience and Glycotechnology 27 (157), E15-E19, 2015

  FCCA(Forum: Carbohydrates Coming of Age)

Keywords

• glycoprotein
• glycopeptide
• thioester method
• chemoenzymatic synthesis
• Endo-M

Details

• CRID: 1390001204369203328
• NII Article ID: 130005099230
• NII Book ID: AA10995236
• DOI: 10.4052/tigg.1414.4e
• ISSN: 18832113
• ISSN: 09157352
• NDL BIB ID: 027138430
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027138430
• Web Site: https://www.jstage.jst.go.jp/article/tigg/27/157/27_1414.4E/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed