Graphene Oxide-based Amplified Fluorescence Sensor for Nucleic Acid Detection through Target-catalyzed Hairpin Assembly

• Yusuke Kitamura

  Department of Applied Chemistry and Biochemistry, Kumamoto University

• Takaaki Miyahata

  Department of Applied Chemistry and Biochemistry, Kumamoto University

• Hirotaka Matsuura

  Department of Applied Chemistry and Biochemistry, Kumamoto University

• Kazuto Hatakeyama

  Department of Applied Chemistry and Biochemistry, Kumamoto University

• Takaaki Taniguchi

  CREST, Japan Science and Technology Agency

• Michio Koinuma

  Department of Applied Chemistry and Biochemistry, Kumamoto University

• Yasumichi Matsumoto

  Department of Applied Chemistry and Biochemistry, Kumamoto University

• Toshihiro Ihara

  Department of Applied Chemistry and Biochemistry, Kumamoto University

抄録

The fluorescence signal of a DNA sensor based on strand exchange on graphene oxide was amplified using a DNA circuit that consisted of autonomous cyclic reactions catalyzed by the target. Selectivity for the target was retained after signal amplification. The fluorescence signal was enhanced ca. 50–100-fold at sub-nanomolar target concentrations under isothermal conditions at 35 °C.

収録刊行物

• Chemistry Letters

  Chemistry Letters 44 (10), 1353-1355, 2015-07-16

  Oxford University Press (OUP)

キーワード

• General Chemistry

詳細情報

• CRID: 1360565169059130624
• NII論文ID: 130005101443
• DOI: 10.1246/cl.150564
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/44/10/1353/55638993/cl.150564.pdf
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