Graphene Oxide-based Amplified Fluorescence Sensor for Nucleic Acid Detection through Target-catalyzed Hairpin Assembly

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Author(s)

    • Kitamura Yusuke
    • Department of Applied Chemistry and Biochemistry, Kumamoto University|CREST, Japan Science and Technology Agency
    • Taniguchi Takaaki
    • CREST, Japan Science and Technology Agency|International Center for Materials Nanoarchitectonics, National Institute for Materials Science
    • Koinuma Michio
    • Department of Applied Chemistry and Biochemistry, Kumamoto University|CREST, Japan Science and Technology Agency
    • Matsumoto Yasumichi
    • Department of Applied Chemistry and Biochemistry, Kumamoto University|CREST, Japan Science and Technology Agency
    • Ihara Toshihiro
    • Department of Applied Chemistry and Biochemistry, Kumamoto University|CREST, Japan Science and Technology Agency

Abstract

The fluorescence signal of a DNA sensor based on strand exchange on graphene oxide was amplified using a DNA circuit that consisted of autonomous cyclic reactions catalyzed by the target. Selectivity for the target was retained after signal amplification. The fluorescence signal was enhanced ca. 50–100-fold at sub-nanomolar target concentrations under isothermal conditions at 35 °C.

Journal

  • Chemistry Letters

    Chemistry Letters 44(10), 1353-1355, 2015

    The Chemical Society of Japan

Codes

  • NII Article ID (NAID)
    130005101443
  • Text Lang
    ENG
  • ISSN
    0366-7022
  • Data Source
    J-STAGE 
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