Zinc Ion-binding Activity of an Anti-ZnO VHH Antibody, 4F2

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Author(s)

    • Sasaki Ryosuke
    • Graduate School of Science and Engineering, Yamagata University
    • Kitahara Ryo
    • College of Pharmaceutical Sciences, Ritsumeikan University
    • Nakazawa Hikaru
    • Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
    • Kumagai Izumi
    • Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
    • Umetsu Mitsuo
    • Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
    • Makabe Koki
    • Graduate School of Science and Engineering, Yamagata University

Abstract

The interaction between biomolecules and inorganic materials has gained much interest for the development of sensors and highly controlled materials in scale and composition. For this purpose, 4F2, a VHH antibody with high affinity and specificity toward zinc oxide (ZnO) surfaces has been constructed. Here, we report that 4F2 also has an affinity toward zinc ion (Zn<sup>2+</sup>). The stability of 4F2 and its Zn<sup>2+</sup> complexes was determined using differential scanning calorimetry. We then determined the binding affinity between 4F2 and Zn<sup>2+</sup> by using isothermal calorimetry. To further elucidate the molecular binding mechanism of 4F2 toward Zn<sup>2+</sup>, we applied the <sup>1</sup>H–<sup>15</sup>N heteronuclear single quantum coherence NMR technique. We found that 4F2 recognized Zn<sup>2+</sup> near the complementarity determining region 3 (CDR3). Our results provide the basis for the recognition mechanism of ZnO by 4F2.

Journal

  • Chemistry Letters

    Chemistry Letters 44(10), 1309-1311, 2015

    The Chemical Society of Japan

Codes

  • NII Article ID (NAID)
    130005101454
  • Text Lang
    ENG
  • ISSN
    0366-7022
  • Data Source
    J-STAGE 
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