Obligatory Role of Early Ca<sup>2+</sup> Responses in H<sub>2</sub>O<sub>2</sub>-Induced β-Cell Apoptosis

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  • Sato Taiji
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka
  • Kaneko Yukiko K.
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka
  • Sawatani Toshiaki
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka
  • Noguchi Akiko
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka
  • Ishikawa Tomohisa
    Department of Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka

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  • Obligatory Role of Early Ca²⁺ Responses in H₂O₂-Induced β-Cell Apoptosis

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Abstract

Our previous study using apoptosis analysis suggested that Ca2+ release through inositol 1,4,5-trisphosphate (IP3) receptors and the subsequent Ca2+ influx through store-operated channels (SOCs) constitute a triggering signal for H2O2-induced β-cell apoptosis. In the present study, we further examined the obligatory role of early Ca2+ responses in β-cell apoptosis induction. H2O2 induced elevation of the cytosolic Ca2+ concentration ([Ca2+]c) consisting of two phases: an initial transient [Ca2+]c elevation within 30 min and a slowly developing one thereafter. The first phase was almost abolished by 2-aminoethoxydiphenylborate (2-APB), which blocks IP3 receptors and cation channels including SOCs, while the second phase was only partially inhibited by 2-APB. The inhibition by 2-APB of the second phase was not observed when 2-APB was added 30 min after the treatment with H2O2. 2-APB also largely inhibited elevation of the mitochondrial Ca2+ concentration ([Ca2+]m) induced by H2O2 when 2-APB was applied simultaneously with H2O2, but not when applied 30 min after H2O2 application. In addition, 2-APB inhibited the release of mitochondrial cytochrome c to the cytosol induced by H2O2 when 2-APB was applied simultaneously with H2O2 but not 30 min post-treatment. H2O2-induced [Ca2+]m elevation and cell death were not inhibited by Ru360, an inhibitor of the mitochondrial calcium uniporter (MCU). These results suggest that the H2O2-induced initial [Ca2+]c elevation, occurring within 30 min and mediated by Ca2+ release through IP3 receptors and subsequent Ca2+ influx through SOCs, leads to [Ca2+]m elevation, possibly through a mechanism independent of MCU, thereby inducing cytochrome c release and consequent apoptosis.

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