Pluripotent cell derivation from male germline cells by suppression of <i>Dmrt1</i> and <i>Trp53</i>

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Diploid germ cells are thought to have pluripotency potential. We recently described a method to derive pluripotent stem cells (PSCs) from cultured spermatogonial stem cells (SSCs) by depleting <i>Trp53</i> and <i>Dmrt1</i>, both of which are known suppressors of teratomas. In this study, we used this technique to analyze the effect of this protocol in deriving PSCs from the male germline at different developmental stages. We collected primordial germ cells (PGCs), gonocytes and spermatogonia, and the cells were transduced with lentiviruses expressing short hairpin RNA against <i>Dmrt1</i> and/or <i>Trp53</i>. We found that PGCs are highly susceptible to reprogramming induction and that only <i>Trp53</i> depletion was sufficient to induce pluripotency. In contrast, gonocytes and spermatogonia were resistant to reprogramming by double knockdown of <i>Dmrt1</i> and <i>Trp53</i>. PSCs derived from PGCs contributed to chimeras produced by blastocyst injection, but some of the embryos showed placenta-only phenotypes suggestive of epigenetic abnormalities of PGC-derived PSCs. These results show that PGCs and gonocytes/spermatogonia have distinct reprogramming potential and also suggest that fresh and cultured SSCs do not necessarily have the same properties.

収録刊行物

  • 家畜繁殖研究會誌

    家畜繁殖研究會誌 61(5), 473-484, 2015

    日本繁殖生物学会

各種コード

  • NII論文ID(NAID)
    130005104118
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    journal article
  • ISSN
    0916-8818
  • NDL 記事登録ID
    026813238
  • NDL 請求記号
    Z54-H305
  • データ提供元
    NDL  IR  J-STAGE 
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