In situ Synthesis and Immobilization of Enzyme Molecules on Microreactor Array Chips

DOI Web Site Web Site 1 Citations 3 References Open Access
  • Ueno Shingo
    Department of Bioengineering, School of Engineering, The University of Tokyo
  • Hirai Tatsunori
    Department of Bioengineering, School of Engineering, The University of Tokyo
  • Sato Shusuke
    Department of Bioengineering, School of Engineering, The University of Tokyo
  • Biyani Manish
    Department of Bioengineering, School of Engineering, The University of Tokyo
  • Kuramochi Hiromi
    Department of Bioengineering, School of Engineering, The University of Tokyo
  • Iizuka Ryo
    Graduate School of Pharmaceutical Sciences, The University of Tokyo
  • Akagi Takanori
    Department of Bioengineering, School of Engineering, The University of Tokyo
  • Funatsu Takashi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo
  • Ichiki Takanori
    Department of Bioengineering, School of Engineering, The University of Tokyo

Search this article

Abstract

The improved catalytic activity of enzymes is required in various fields. Enzymes have conventionally been improved by the screening of bacteria possessing mutant enzymes. However, the screening conditions are limited since screening requires the growth of bacteria. Here, we report the development of a protein microarray for the analysis of enzymatic activity. A his-tagged enzyme is synthesized in situ and immobilized on the microarray, which is composed of microreactors with a diameter and depth of 4 μm and a density of 1.0 x 106 reactors/cm2. β-glucosidase, synthesized in situ using a cell-free synthesis system, was immobilized on the microreactor array chip and its catalytic activity was observed. This enzyme-immobilized microarray is expected to enable the rapid and quantitative screening of enzymes.

Journal

Citations (1)*help

See more

References(3)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top