細菌べん毛モーター固定子の組込みと活性化機構

• 今田 勝巳

  大阪大学大学院理学研究科

• 小嶋 誠司

  名古屋大学大学院理学研究科

書誌事項

タイトル別名

• Structural Basis of the Assembly and Activation Mechanism of the Bacterial Flagellar Stator Complex
• サイキン ベンモウ モーター コテイシ ノ クミコミ ト カッセイカ キコウ

抄録

Bacteria swim in aqueous environment by rotating the flagellum driven by a reversible motor embedded in the cell membrane. The torque of the motor is generated by the rotor-stator interaction coupled with the ion flow through the stator. A dozen of stators assemble around the rotor and are fixed to the peptidoglycan layer. The ion-conductivity of the stator is coupled to its assembly, and proper anchoring of the stator is required for torque generation. We determined the crystal structures of C-terminal fragments of the B-subunit of the stator including the region responsible for anchoring the stator, and performed the functional analyses using the mutants designed based on the structure. These studies revealed that the conformational change of the N-terminal region of the fragment is essential to form a functional stator around the rotor.

収録刊行物

• 日本結晶学会誌

  日本結晶学会誌 57 (5), 291-296, 2015

  日本結晶学会

詳細情報

• CRID: 1390001204090052736
• NII論文ID: 130005105684
• NII書誌ID: AN00188364
• DOI: 10.5940/jcrsj.57.291
• ISSN: 18845576
• ISSN: 03694585
• NDL書誌ID: 026850521
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026850521
• Web Site: https://www.jstage.jst.go.jp/article/jcrsj/57/5/57_291/_pdf
• 本文言語コード: ja
• データソース種別:
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  • NDL
  • Crossref
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  • KAKEN
• 抄録ライセンスフラグ: 使用不可