Maternal molecular hydrogen administration on lipopolysaccharide-induced mouse fetal brain injury

• Nakano Tomoko

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Kotani Tomomi

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Mano Yukio

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Tsuda Hiroyuki

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Imai Kenji

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Ushida Takafumi

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Li Hua

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Miki Rika

  Bio-database Institute of Reproductive and Developmental Medicine, Nagoya University Graduate School of Medicine

• Sumigama Seiji

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Sato Yoshiaki

  Division of Neonatology, Center for Maternal-Neonatal Care, Nagoya University Hospital

• Iwase Akira

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

• Hirakawa Akihiro

  Biostatistics Section, Center for Advanced Medicine and Clinical Research Cancer, Nagoya University Graduate School of Medicine

• Asai Masato

  Department of Pathology, Nagoya University Graduate School of Medicine

• Toyokuni Shinya

  Pathology and Biological Responses, Nagoya University Graduate School of Medicine

• Kikkawa Fumitaka

  Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine

Abstract

Fetal brain injury is often related to prenatal inflammation; however, there is a lack of effective therapy. Recently, molecular hydrogen (H₂), a specific antioxidant to hydroxyl radical and peroxynitrite, has been reported to have anti-inflammatory properties. The aim of this study was to investigate whether maternal H₂ administration could protect the fetal brain against inflammation. Pregnant C3H/HeN mice received an intraperitoneal injection of lipopolysaccharide (LPS) on gestational day 15.5 and were provided with H₂ water for 24 h prior to LPS injection. Pup brain samples were collected on gestational day 16.5, and the levels of apoptosis and oxidative damage were evaluated using immunohistochemistry. Interleukin-6 (IL-6) levels were examined using real-time PCR. The levels of apoptosis and oxidative damage, as well as the levels of IL-6 mRNA, increased significantly when the mother was injected with LPS than that in the control group. However, these levels were significantly reduced when H₂ was administered prior to the LPS-injection. Our results suggest that LPS-induced apoptosis, oxidative damage and inflammation in the fetal brain were ameliorated by maternal H₂ administration. Antenatal H₂ administration might protect the premature brain against maternal inflammation.

Journal

• Journal of Clinical Biochemistry and Nutrition

  Journal of Clinical Biochemistry and Nutrition 57 (3), 178-182, 2015

  SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Keywords

• molecular hydrogen
• premature infant
• anti-oxidant
• anti-inflammation
• brain injury

Details

• CRID: 1390282679648738304
• NII Article ID: 130005106454
• DOI: 10.3164/jcbn.15-90
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://www.jstage.jst.go.jp/article/jcbn/57/3/57_15-90/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016232525
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed