Increased Expression of Osteopontin in the Degenerating Striatum of Rats Treated with Mitochondrial Toxin 3-Nitropropionic Acid: A Light and Electron Microscopy Study
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- Kim Hong-Lim
- Department of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University Integrative Research Support Center, College of Medicine, Catholic University
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- Lee Mun-Yong
- Department of Anatomy, College of Medicine, Catholic University
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- Shin Yoo-Jin
- Department of Anatomy, College of Medicine, Catholic University
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- Song Doo-Won
- Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University
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- Park Jieun
- Integrative Research Support Center, College of Medicine, Catholic University
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- Chang Byung-Soo
- Department of Cosmetology, Hanseo University
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- Lee Jong-Hwan
- Department of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University
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The mycotoxin 3-nitropropionic acid (3NP) is an irreversible inhibitor that induces neuronal damage by inhibiting mitochondrial complex II. Neurodegeneration induced by 3NP, which is preferentially induced in the striatum, is caused by an excess influx and accumulation of calcium in mitochondria. Osteopontin (OPN) is a glycosylated phosphoprotein and plays a role in the regulation of calcium precipitation in the injured brain. The present study was designed to examine whether induction of OPN protein is implicated in the pathogenesis of 3NP-induced striatal neurodegeneration. We observed overlapping regional expression of OPN, the neurodegeneration marker Fluoro-Jade B, and the microglial marker ionized calcium-binding adaptor molecule 1 (Iba1) in the 3NP-lesioned striatum. OPN expression was closely associated with the mitochondrial marker NADH dehydrogenase (ubiquinone) flavoprotein 2 in the damaged striatum. In addition, immunoelectron microscopy demonstrated that OPN protein was specifically localized to the inner membrane and matrix of the mitochondria in degenerating striatal neurons, and cell fragments containing OPN-labeled mitochondria were also present within activated brain macrophages. Thus, our study revealed that OPN expression is associated with mitochondrial dysfunction produced by 3NP-induced alteration of mitochondrial calcium homeostasis, suggesting that OPN is involved in the pathogenesis of striatal degeneration by 3NP administration.
収録刊行物
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- Acta Histochemica et Cytochemica
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Acta Histochemica et Cytochemica 48 (5), 135-143, 2015
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詳細情報 詳細情報について
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- CRID
- 1390282679838040448
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- NII論文ID
- 130005106596
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- ISSN
- 13475800
- 00445991
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可