The combined effect of clothianidin and environmental stress on the behavioral and reproductive function in male mice

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Author(s)

    • HIRANO Tetsushi HIRANO Tetsushi
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • YANAI Shogo AIHARA Yoshiko
    • Laboratory of Food and Nutritional Chemistry, Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • FURUYASHIKI Tomoyuki
    • Department of Biochemistry and Molecular Biology, Graduate School of Medicine, Kobe University, Kobe, Hyogo, 650–0017, Japan
    • MANTANI Youhei
    • Laboratory of Histophysiology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • YOKOYAMA Toshifumi
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • KITAGAWA Hiroshi
    • Laboratory of Histophysiology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • HOSHI Nobuhiko
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • YANAI Shogo
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • OMOTEHARA Takuya
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • HASHIMOTO Rie
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • UMEMURA Yuria
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • KUBOTA Naoto
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • MINAMI Kiichi
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • NAGAHARA Daichi
    • Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan
    • MATSUO Eiko
    • Laboratory of Microbiology and Immunology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657–8501, Japan

Abstract

Neonicotinoids, some of the most widely used pesticides in the world, act as agonists to the nicotinic acetylcholine receptors (nAChRs) of insects, resulting in death from abnormal excitability. Neonicotinoids unexpectedly became a major topic as a compelling cause of honeybee colony collapse disorder, which is damaging crop production that requires pollination worldwide. Mammal nAChRs appear to have a certain affinity for neonicotinoids with lower levels than those of insects; there is thus rising concern about unpredictable adverse effects of neonicotinoids on vertebrates. We hypothesized that the effects of neonicotinoids would be enhanced under a chronic stressed condition, which is known to alter the expression of targets of neonicotinoids, <i>i.e.</i>, neuronal nAChRs. We performed immunohistochemical and behavioral analyses in male mice actively administered a neonicotinoid, clothianidin (CTD; 0, 10, 50 and 250 mg/kg/day), for 4 weeks under an unpredictable chronic stress procedure. Vacuolated seminiferous epithelia and a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 were observed in the testes of the CTD+stress mice. In an open field test, although the locomotor activities were not affected, the anxiety-like behaviors of the mice were elevated by both CTD and stress. The present study demonstrates that the behavioral and reproductive effects of CTD become more serious in combination with environmental stress, which may reflect our actual situation of multiple exposure.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 77(10), 1207-1215, 2015

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130005107605
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    026822126
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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