Affinity Isolation of Desired Restriction Fragment from Human Genome Using Double-duplex Invasion of Biotin-bound Pseudo-complementary PNA

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Author(s)

    • Shigi Narumi
    • Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba
    • Wang Xiaohui
    • Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba
    • Kunifuda Hiroko
    • Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba
    • Sumaoka Jun
    • Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba|Department of Applied Chemistry, School of Engineering, Tokyo University of Technology
    • Komiyama Makoto
    • Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba

Abstract

A predetermined fragment in the human genome was successfully isolated by affinity separation using biotin–streptavidin interaction. The whole genome from human cells was first digested by restriction enzymes and then treated with a pair of pseudo-complementary peptide nucleic acids (pcPNAs). A biotin was covalently bound to either of the pcPNAs. With the use of streptavidin-coupled resin, the targeted fragment involving the invasion site of the pcPNAs was selectively picked up from the genomic digests in high yields.

Journal

  • Chemistry Letters

    Chemistry Letters 44(11), 1569-1571, 2015

    The Chemical Society of Japan

Codes

  • NII Article ID (NAID)
    130005108088
  • Text Lang
    ENG
  • ISSN
    0366-7022
  • Data Source
    J-STAGE 
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