Affinity Capillary Electrophoresis for Selective Control of Electrophoretic Mobility of Sialic Acid Using Lanthanide–Hexadentate Macrocyclic Polyazacarboxylate Complexes

• GOTO Daiki

  Graduate School of Science and Engineering, Saitama University

• OUCHI Kazuki

  Graduate School of Science and Engineering, Saitama University

• SHIBUKAWA Masami

  Graduate School of Science and Engineering, Saitama University

• SAITO Shingo

  Graduate School of Science and Engineering, Saitama University

Abstract

It is difficult to control the electrophoretic mobility in order to obtain high resolution among saccharides in complex samples. We report herein on a new affinity capillary electrophoresis (ACE) method for an anionic monosaccharide, N-acetylneuraminic acid (Neu5Ac), which is important in terms of pathological diagnosis, using lanthanide-hexadentate macrocyclic polyazacarboxylate complexes (Ln-NOTA) as affinity reagents. It was shown that Ln-NOTA complexes increased the anionic mobility of Neu5Ac by approximately 40% through selective complexation with Neu5Ac. The extent of change in the mobility strongly depended on the type of central metal ion of Ln-NOTA. The stability constant (K) of Lu-NOTA with Neu5Ac was determined by ACE to be log K_b = 3.62 ± 0.04, which is the highest value among artificial receptors for Neu5Ac reported so far. Using this ACE, the Neu5Ac content in a glycoprotein sample, α1-acid glycoprotein (AGP), was determined after acid hydrolysis. Complete separation between Neu5Ac and hydrolysis products was successful by controlling the mobility to determine the concentration of Neu5Ac.

Journal

• Analytical Sciences

  Analytical Sciences 31 (11), 1143-1149, 2015

  The Japan Society for Analytical Chemistry

Keywords

• Affinity capillary electrophoresis
• sialic acid
• lanthanide complexes

Details

• CRID: 1390282679236220928
• NII Article ID: 130005108607
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.31.1143
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 026859203
• PubMed: 26561258
• Web Site: http://id.ndl.go.jp/bib/026859203
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026859203
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.1143.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.1143/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/11/31_1143/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed