Quantification of (−)-epigallocatechin-3-gallate Inhibition of Migration and Invasion of Oral Squamous Cell Carcinoma Cell Lines Using Real-time Cell Analysis

• EGAWA Shunya

  Department of Pharmacology, Showa University School of Medicine Department of Otorhinolaryngology, Showa University School of Medicine

• IWAI Shinichi

  Department of Pharmacology, Showa University School of Medicine Department of Healthcare and Regulatory Sciences, Showa University School of Pharmacy

• IIJIMA Kentaro

  Department of Pharmacology, Showa University School of Medicine

• TOJU Akiko

  Department of Pharmacology, Showa University School of Medicine

• YAMAMOTO Ran

  Department of Pharmacology, Showa University School of Medicine

• HAYASHI Takeshi

  Department of Pharmacology, Showa University School of Medicine Department of Otorhinolaryngology, Showa University School of Medicine

• SAMBE Takehiko

  Department of Pharmacology, Showa University School of Medicine

• OGUCHI Katsuji

  Department of Pharmacology, Showa University School of Medicine

Bibliographic Information

Other Title

• Quantification of (−)-epigallocatechin-3-gallate Inhibition of Migration and Invasion of Oral Squamous Cell Carcinoma Cell Lines Using Real-time Cell Analysis

Abstract

Catechins found in green tea, in particular (−)-epigallocatechin-3-gallate (EGCG), have antitumor activity. The primary antitumor actions of catechins are anti-oxidative, anti-angiogenic, and anti-metastatic effects. Cell migration and invasion contribute to the metastatic potential of tumors. Real-time cell analysis (RTCA) measures cell migration and invasion in vitro. In the present study, using RTCA, we investigated whether the cell migration and invasion of oral squamous cell carcinomas (OSCCs) of the tongue and floor of the mouth were inhibited by EGCG. Studies were performed using the human SCC-4 and SAS cell lines, which are poorly differentiated OSCCs of the tongue, and the HO-1-u-1 cell line, an OSCC of the floor of the mouth. SCC-4 cells exhibited high cell migration and invasion compared with the SAS and HO-1-u-1 cells. EGCG was most effective in inhibiting the migration and invasion of SCC-4 cells, and inhibited OSCC cell invasion more strongly than it inhibited cell migration. EGCG inhibited the expression of matrix metalloproteinase (MMP)-2, MMP-9, and integrin α1 and β1 mRNA in the OSCC cell lines, particularly SCC-4 cells. The findings of the present study suggest that EGCG inhibits OSCC cell migration and invasion by inhibiting MMP-2, MMP-9, and integrin α1 and β1 expression. Thus, EGCG may be a suitable agent or lead compound for the inhibition of OSCC metastasis.

Journal

• The Showa University Journal of Medical Sciences

  The Showa University Journal of Medical Sciences 27 (2), 71-81, 2015

  The Showa University Society

Keywords

• oral squamous cell carcinoma
• migration
• invasion
• (−)-epigallocatechin-3-gallate (EGCG)
• real-time cell analyzer

Details

• CRID: 1390001204373716224
• NII Article ID: 130005110364
• NII Book ID: AA10781651
• DOI: 10.15369/sujms.27.71
• ISSN: 21850968
• ISSN: 09156380
• Web Site: http://id.nii.ac.jp/1670/00000571/
• Web Site: https://www.jstage.jst.go.jp/article/sujms/27/2/27_71/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016275262
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed