Methylmercury induces release of a cytotoxic factor from HEK293 cells into medium

• Toyama Takashi

  Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Murakami Souichi

  Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Kuge Shusuke

  Department of Microbiology, Tohoku Pharmaceutical University

• Hwang Gi-Wook

  Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Naganuma Akira

  Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University

抄録

HEK293 cells were cultured in medium containing methylmercury (MeHg), followed by replacement with MeHg-free medium and further culturing. Thus, MeHg preconditioning medium (MeHg-PM) were obtained. Untreated HEK293 cells and C17.2 cells (mouse neural stem cells) were placed in the obtained MeHg-PM for culturing, which resulted in significantly inhibited cell growth. This cell growth inhibition was not affected by heating or proteinase K treatment, suggesting that neither proteins nor peptides caused the growth inhibition.

収録刊行物

• Fundamental Toxicological Sciences

  Fundamental Toxicological Sciences 2 (5), 223-226, 2015

  一般社団法人 日本毒性学会

キーワード

• Methylmercury
• Toxicity
• HEK 293 cells
• C17.2 cells

詳細情報

• CRID: 1390001205764231808
• NII論文ID: 130005113420
• DOI: 10.2131/fts.2.223
• ISSN: 2189115X
• Web Site: https://www.jstage.jst.go.jp/article/fts/2/5/2_223/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2017000395
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可