Evaluation of genetic variation among wild rice populations in Cambodia

• Orn Chhourn

  Graduate School of Agricultural Science, Kobe University Cambodian Agricultural Research and Development Institute (CARDI)

• Shishido Rieko

  College of Bioresource Sciences, Nihon University

• Akimoto Masahiro

  Obihiro University of Agriculture and Veterinary Medicine

• Ishikawa Ryo

  Graduate School of Agricultural Science, Kobe University

• Htun Than Myint

  Graduate School of Agricultural Science, Kobe University

• Nonomura Ken-Ichi

  National Institute of Genetics

• Koide Yohei

  Hakubi Center for Advanced Research/Graduate School of Agriculture, Kyoto University

• Sarom Men

  Royal University of Agriculture (RUA)

• Vang Seng

  Cambodian Agricultural Research and Development Institute (CARDI)

• Sophany Sakhan

  Cambodian Agricultural Research and Development Institute (CARDI)

• Makara Ouk

  Cambodian Agricultural Research and Development Institute (CARDI)

• Ishii Takashige

  Graduate School of Agricultural Science, Kobe University

抄録

A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources.

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 65 (5), 430-437, 2015

  日本育種学会

キーワード

• wild rice population
• <i>Oryza rufipogon</i> Griff.
• genetic variation
• Cambodia
• annual
• perennial

詳細情報

• CRID: 1390001204722283136
• NII論文ID: 130005114134
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.65.430
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 026989042
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026989042
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/65/5/65_430/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可