Mesenchymal stem cells ameliorate intra-amniotic inflammation-related neonatal complications in rats

• Honda Izumi

  Department of Comprehensive Reproductive Medicine, Tokyo Medical and Dental University Graduate School

• Taki Atsuko

  Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School

• Morioka Chikako

  Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School

• Komaki Motohiro

  Department of Nanomedicine (DNP), Tokyo Medical and Dental University Graduate School

• Miyasaka Naoyuki

  Department of Comprehensive Reproductive Medicine, Tokyo Medical and Dental University Graduate School

• Oshima Noriko

  Department of Comprehensive Reproductive Medicine, Tokyo Medical and Dental University Graduate School

• Iseki Sachiko

  Department of Molecular Craniofacial Embryology, Tokyo Medical and Dental University Graduate School

• Morio Tomohiro

  Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University Graduate School

• Kubota Toshiro

  Department of Comprehensive Reproductive Medicine, Tokyo Medical and Dental University Graduate School

• Morita Ikuo

  Department of Cellular Physiological Chemistry, Tokyo Medical and Dental University Graduate School

Abstract

Objective: The aim of this study was to establish a novel rat model of neonatal complications secondary to intra-amniotic infection/inflammation in order to investigate the therapeutic efficacy of rat umbilical cord-derived mesenchymal stem cells (rUCMSCs).<BR>Methods: On gestational age day (GAD) 16, approximately 0.2 μg of lipopolysaccharide (LPS) was directly injected into the amniotic cavity of a pregnant rat. Placental inflammation on GAD 20 was histologically evaluated and the cytokine (Il-1β, Tnfα, Mcp-1, Il-6, Cxcl-1) and prostaglandin synthesis enzyme (Cox-1, Cox-2) expression patterns were analyzed by quantitative real-time polymerase chain reaction. Neonatal lung and brain injuries on postnatal day (PND) 14 were assessed histologically. rUCMSCs were injected intravenously into pups to investigate their therapeutic efficacy.<BR>Results: LPS significantly decreased alive-birth rates. Significant increases in inflammatory cell infiltration and up-regulation of Mcp-1 and Cox-2 expression were observed in the placenta. In the neonates, the areas staining positive for myelin basic protein in the brain and radial alveolar counts in the lungs were significantly reduced in the LPS group compared with the control group. rUCMSCs improved myelination and alveolarization.<BR>Conclusion: Intrauterine injection of LPS causes placental inflammation along with neonatal brain and lung injuries in neonatal rats. Postnatal administration of rUCMSCs alleviates these neonatal complications.

Journal

• Inflammation and Regeneration

  Inflammation and Regeneration 35 (5), 261-268, 2015

  The Japanese Society of Inflammation and Regeneration

Keywords

• chorioamnionitis
• periventricular leukomalacia
• bronchopulmonary dysplasia
• preterm birth
• fetal inflammatory response syndrome

Details

• CRID: 1390282680235043840
• NII Article ID: 130005114585
• DOI: 10.2492/inflammregen.35.261
• ISSN: 18808190
• ISSN: 18809693
• Web Site: https://www.jstage.jst.go.jp/article/inflammregen/35/5/35_261/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016324934
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed