酵素反応を用いた匂いカメラによる手掌部のエタノールガスの可視化計測

DOI Web Site 参考文献4件
  • IITANI Kenta
    Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • SATO Toshiyuki
    Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • WANG Xin
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • TOMA Koji
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • ARAKAWA Takahiro
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • MITSUBAYASHI Kohji
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

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タイトル別名
  • “Sniffer-camera” using enzyme reaction for visualization of transpired ethanol from palm skin

抄録

An imaging system for gaseous ethanol transpired from human palm skin, based on an enzymatic reaction, was assembled and validated. This system uses a highly sensitive camera that measures gaseous ethanol concentrations as intensities of chemiluminescence from luminol’s reaction induced by alcohol oxidase (AOD) and a luminol-hydrogen peroxide-horseradish peroxidase (HRP) system. Conversion of gaseous ethanol concentrations and distributions proceed on an enzyme-immobilized mesh substrate with luminol solution in a dark box. In order to visualize ethanol transpired from human palm skin, we improved the chemiluminescence sensitivity of the imaging system with a mixture of a high-purity luminol solution of luminol sodium salt HG solution and an enhancer of eosin Y solution. The detection limit of the ethanol concentration was 4.9 ppm. This highly sensitive imaging allows successful visualization of ethanol transpired from palm skin.

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