<i>Burkholderia</i> of Plant-Beneficial Group are Symbiotically Associated with Bordered Plant Bugs (Heteroptera: Pyrrhocoroidea: Largidae)

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Author(s)

    • TAKESHITA KAZUTAKA Takeshita Kazutaka
    • Graduate School of Agriculture, Hokkaido University|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center
    • Navarro Ronald
    • Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
    • Hori Tomoyuki
    • Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
    • Sone Teruo
    • Graduate School of Agriculture, Hokkaido University
    • Kamagata Yoichi
    • Graduate School of Agriculture, Hokkaido University|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center
    • Mergaert Peter
    • Graduate School of Agriculture, Hokkaido University|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center|Institute for Integrative Biology of the Cell, Centre National de la Recherche Scientifique (CNRS)
    • Kikuchi Yoshitomo
    • Graduate School of Agriculture, Hokkaido University|Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center

Abstract

A number of phytophagous stinkbugs (order Heteroptera: infraorder Pentatomomorpha) harbor symbiotic bacteria in a specific midgut region composed of numerous crypts. Among the five superfamilies of the infraorder Pentatomomorpha, most members of the Coreoidea and Lygaeoidea are associated with a specific group of the genus <i>Burkholderia</i>, called the "stinkbug-associated beneficial and environmental (SBE)" group, which is not vertically transmitted, but acquired from the environment every host generation. A recent study reported that, in addition to these two stinkbug groups, the family Largidae of the superfamily Pyrrhocoroidea also possesses a <i>Burkholderia</i> symbiont. Despite this recent finding, the phylogenetic position and biological nature of <i>Burkholderia</i> associated with Largidae remains unclear. Based on the combined results of fluorescence <i>in situ</i> hybridization, cloning analysis, Illumina deep sequencing, and egg inspections by diagnostic PCR, we herein demonstrate that the largid species are consistently associated with the "plant-associated beneficial and environmental (PBE)" group of <i>Burkholderia</i>, which are phylogenetically distinct from the SBE group, and that they maintain symbiosis through the environmental acquisition of the bacteria. Since the superfamilies Coreoidea, Lygaeoidea, and Pyrrhocoroidea are monophyletic in the infraorder Pentatomomorpha, it is plausible that the symbiotic association with <i>Burkholderia</i> evolved at the common ancestor of the three superfamilies. However, the results of this study strongly suggest that a dynamic transition from the PBE to SBE group, or <i>vice versa</i>, occurred in the course of stinkbug evolution.

Journal

  • Microbes and Environments

    Microbes and Environments 30(4), 321-329, 2015

    Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology

Codes

  • NII Article ID (NAID)
    130005116033
  • NII NACSIS-CAT ID (NCID)
    AA11551577
  • Text Lang
    ENG
  • ISSN
    1342-6311
  • NDL Article ID
    027072974
  • NDL Call No.
    Z54-J644
  • Data Source
    NDL  J-STAGE 
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