Cellular Uptake of IgG Using Collagen-Like Cell-Penetrating Peptides

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Author(s)

    • Masuda Ryo Masuda Ryo
    • Department of Chemistry and Biochemistry, School of Advanced Science and Engineering, Waseda University|Research Institute for Science and Engineering, Waseda University

Abstract

Cell-penetrating peptides (CPPs) are attractive tools for delivering macromolecules that have poor membrane permeability, such as antibodies, into cells. However, the major drawback of conventional CPPs is their instability in bodily fluids. We previously reported a novel CPP employing a collagen-like triple-helical structure that exhibited remarkable resistance against serum proteases. Herein, we report the delivery of full-length immunoglobulin G (IgG) antibody into cells using a triple-helical CPP. The CPP was conjugated to IgG <i>via</i> a one-pot reaction using 2-iminothiolane as a crosslinking reagent. The triple-helical CPP was less prone to being aggregated and neutralized by serum than was octaarginine, a conventional CPP. However, most of the conjugates were found to be entrapped in endosomes.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 39(1), 130-134, 2016

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    130005116494
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • ISSN
    0918-6158
  • NDL Article ID
    027013151
  • NDL Call No.
    Z53-V41
  • Data Source
    NDL  J-STAGE 
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